Characteristics of Crassostrea Oyster Cytochrome Oxidase Subunit I (Coi) Gene As Species Identity In Delta Cimanuk, West Java
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Crassostrea,
Delta Cimanuk,
molecular identification,
morphology
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Abstract
Oysters belong to the Crassostrea, which is a type of shellfish that lives as benthos in waters under the same substrate and environmental conditions will exhibit similar morphological changes in response. There are many types of oysters that have a similar shape are often an obstacle to differentiate of Crassostrea species through morphological identification. The purpose of this research was to identify the types of oysters found in the waters of Delta Cimanuk through analysis of the morphology and nucleotide diversity of Cytochrome Oxidase subunit I (COI) gene, as basic information on proper management and conservation. The morphological identification results show that there were two species of the Crassostrea. Identification of species through the Barcoding DNA technique shows that there is one type of oyster: Crassostrea iredalei with an accuracy of 99.5%.
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References
Goodwin, K. D., Thompson, L. R., Duarte, B., Kahlke, T., Thompson, A. R., Marques, J. C., Caçador, I. (2017). DNA sequencing as a tool to monitor marine ecological status. Frontiers in Marine Science, 4:1–14. https://doi.org/10.3389/fmars.2017.00107
Hermawati, S., Sulistiono, Samosir, A. M. (2017). Distribution, condition and gonad maturity of the invasive pacific oysters (Crassostrea gigas, Thunberg 1793) in Cimanuk Delta, Indramayu, West Java, Indonesia. Omni-Akuatika, 13(2):99–110.
Hollingsworth, P. M. (2011). Refining the DNA barcode for land plants. Proceedings of the National Academy of Sciences of the United States of America, 108(49):19451–19452. https://doi.org/10.1073/pnas.1116812108
Hsiao, S. T., Chuang, S. C., Chen, K. S., Ho, P. H., Wu, C. L., Chen, C. A. (2016). DNA barcoding reveals that the common cupped oyster in Taiwan is the Portuguese oyster Crassostrea angulata (Ostreoida; Ostreidae), not C. gigas. Scientific Reports, 6:1–11. https://doi.org/10.1038/srep34057
Octavina, C., Yulianda, F., Krisanti, M. (2014). Struktur komunitas tiram daging di perairan estuaria Kuala Gigieng , Kabupaten Aceh Besar , Provinsi Aceh Population structure of oysters in estuary area of Kuala Gigieng , Aceh Besar District , Aceh Province. Depik, 3(2):108–117.
Pereira, J. C., Chaves, R., Bastos, E., Leitão, A., Guedes-Pinto, H. (2011). An efficient method for genomic DNA extraction from different molluscs species. International Journal of Molecular Sciences, 12(11):8086–8095. https://doi.org/10.3390/ijms12118086
Roger, F., Godhe, A., Gamfeldt, L. (2012). Genetic diversity and ecosystem functioning in the face of multiple stressors. PLoS ONE, 7(9):e45007. https://doi.org/10.1371/journal.pone.0045007
Sheth, B. P., Thaker, V. S. (2017). DNA barcoding and traditional taxonomy: an integrated approach for biodiversity conservation. Genome, 6(7):618–628.
Sjöqvist, C. O., Kremp, A. (2016). Genetic diversity affects ecological performance and stress response of marine diatom populations. ISME Journal, 10(11):2755–2766. https://doi.org/10.1038/ismej.2016.44
Slynko, Y. V., Slynko, E. E., Pirkova, A. V., Ladygina, L. V., Ryabushko, V. I. (2018). Mitochondrial DNA barcoding of the pacific oyster Crassostrea gigas (Thunberg, 1793) (Mollusca: Bivalvia: Ostreidae), cultivated in the Black Sea. Russian Journal of Genetics, 54(12):1445–1451. https://doi.org/10.1134/S1022795418120153
Stapley, J., Reger, J., Feulner, P. G. D., Smadja, C., Galindo, J., Ekblom, R., Bennison, C., Ball, A. D., Beckerman, A. P., Slate, J. (2010). Adaptation genomics: The next generation. Trends in Ecology and Evolution, 25(12):705–712. https://doi.org/10.1016/j.tree.2010.09.002
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. (2011). MEGA5 : molecular evolutionary genetics analysis using maximum likelihood , evolutionary distance , and maximum parsimony methods. Molecular Biology and Evolution, 28(10):2731–2739. https://doi.org/10.1093/molbev/msr121